Vaccines are a proven benefit in the world of animal science. People have vaccinated both pets and livestock for decades.
Soon beekeepers may be able to protect their colonies through vaccination. The University of Georgia College of Agricultural and Environmental Sciences (CAES) and Dalan Animal Health have teamed up to advance the world’s first honeybee vaccine.
“You don’t have to look far to know honeybees are having a lot of problems right now. Hives will die unless you intercede with herculean efforts,” said Keith Delaplane, professor in the CAES Department of Entomology and director of the UGA Bee Program.
He wasn’t sure what would come of an initial meeting with Dalan set up by the Athens-Clarke County Economic Development Department, but Delaplane is proud that the company came to Athens and to UGA.
“The timing is right for it to be coming to our college,” Delaplane said.
Annette Kleiser, co-founder and CEO of Dalan, built the company in a virtual format to optimize the creativity of a team of experts based around the world. While the virtual model works for their team, Kleiser acknowledged the need for a “home base” for the company — a space for labs, offices and growth.
“We looked around at all the things we would like to have for an insect health company,” Kleiser said. “We wanted a world-class university with access to talent, with a veterinary school that understood animal vaccines, that is focused on agriculture and sustainable agriculture and that has a bee lab.”
The company also needed a location with a climate where research could be active nine-plus months of the year and, ideally, a culture of entrepreneurship, Kleiser added. The proximity to Atlanta to serve a global product with a global program was an added benefit.
“We made this wish list and there was Athens, Georgia,” Kleiser said. “We visited and met with the economic development folks and they introduced us to many people, including Keith. We saw the enthusiasm for what we were doing — there was an alignment.”
Present at those initial meetings was Chris Rhodes, director of industry partnerships and project-based learning at CAES. Rhodes noted that while sponsored research is not a new concept, Dalan is sponsoring research to create a new industry and is a tenant of the UGA Innovation District.
“Dalan is the first tenant to work with CAES researchers like this,” Rhodes said. “This is exactly why we have the Innovation District, to provide a home for innovative companies who work with faculty and students on groundbreaking commercial solutions.”
Kleiser, who has worked with a number of tech-transfer offices at universities in the U.S. and internationally, said UGA’s willingness to work with the private sector stood out.
“I was very surprised. The invitation to be at the hub, to locate the company in the accelerator, made it so easy,” she said. “I have not come across a university innovation space that invited a private company. The welcoming environment and ability to find solutions quickly was a big surprise and a big plus.”
Depending on the queen
How to vaccinate a honeybee? It won’t come down to tiny syringes. The research is focused on a different delivery method: candy for the queen.
Inherited immunity, according to Delaplane, involves the queen incorporating fragments of bacterial cells into her eggs. The egg contains the antigen, which in turn creates and promotes an immune response. The current project calls for feeding queen bees Dalan’s proprietary vaccine after which the inoculated queen, for the remainder of her lifetime, will produce worker bees that are primed to be immune against that pathogen.
“In a perfect scenario, the queens could be fed a cocktail within a queen candy — the soft, pasty sugar that queen bees eat while in transit,” Delaplane said. “Queen breeders could advertise ‘fully vaccinated queens.’”
There is also some evidence that immunized worker bees could pass immunity to their sisters. As they age, Delaplane said, bees go through a series of predictable tasks — cleaning cells, feeding larvae, processing honey, foraging and guarding the nest. If an immunized nurse bee feeds her larval sisters, it could give them immunity. He likened the relationship to the mother-baby relationship of lactating mammals where immune benefits are transferred through breastmilk.
Solving a global bee pandemic
The diseases seen today in beekeeping are global pandemics, according to Kleiser. Bees are sent worldwide and, while there are strict measures to prevent the spread, containment is impossible.
“This work with Keith is really, really important. One of the reasons is that this work is so new,” Kleiser said. “There are no guidelines, no handbook. We are developing, together with Keith, what will be the gold standard for these trials. It’s really exciting; it is the first of its kind. When you engage with the regulators moving forward, this is what they will look at.”
The team is currently working on American foulbrood (AFB), one of the most fatal of bacterial diseases, Delaplane said. Once they succeed with a vaccine against bacterial disease, he has his eye on an even more complicated problem — tackling the viruses that can easily decimate hives.
“It is trickier to create inherited immunity with viruses, yet viruses are front and center in all literature about bee health problems,” he added. “They have proven to be an intractable problem — my hope is that this partnership can lead to a viral vaccine.”
For Dalan, the partnership with UGA brought another benefit: access to students and alumni.
“There is a real opportunity here for internships,” Kleiser said. “We’ve already had students reach out; we’ve seen the initiative. This is different than an opportunity at a zoo or botanical garden. There’s a biotech company that works with these unique animals and we’re a very young company that allows for creativity.”
Kleiser is excited to not only work with students on internships, but to offer a new, global concept as they consider life after college.
“When students have ideas, they need to know it is possible to turn them into a reality,” she said. “They need the encouragement — and the excitement is there — that it is possible.”
Putting insect health on the map
“People don’t understand how hard it is to keep bees alive,” Delaplane said. “I can’t imagine a more frightening branch of agriculture to be in. It takes ceaseless attention.”
Once released, Delaplane added, these vaccines would be a game-changer for beekeepers who have had few resources except supportive therapies for decades.
“We are putting insect health on the map,” Kleiser said. “It will have a huge impact, not just on what we learn, but opportunities for students and for jobs that don’t currently exist.”
While the potential benefits are concrete, timelines — especially on a totally novel, first-in-class regulated vaccine — are hazier. Dalan is at the final stages of the approval process to obtain a conditional license from the U.S. Department of Agriculture Center for Veterinary Biologics (CVB), according to Kleiser.
“For us, it was important to establish (the honey bee vaccine) as an animal vaccine, just like a chicken or swine or cattle vaccine, that is regulated by the USDA-CVB; they oversee the approval of animal vaccines,” she said. “Once the vaccine is filled in bottles, the final steps in the detailed review process are confirmatory testing by the CVB laboratories for purity, etc. It is difficult to predict the timing, as much is out of our hands, though the CVB has been remarkably adaptive and flexible in our submissions as we go through the registration process for this novel product.”
The team is gearing up for that release ahead of the approaching 2023 honey bee season.